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Anti-CD45 [ITS_rhCD45]-AF555

Anti-CD45 [ITS_rhCD45]-AF555
Product Type: Therapeutics (In Vivo)
Format: Anti-CD45, ITS_rhCD45, mouse variable, mouse IgG1, kappa
Clone ID: [ITS_rhCD45]
RRID: AB_2910538
Common Names: Anti-CD45 [ITS_rhCD45]-AF555 | Anti-CD45 [ITS_rhCD45]-AlexaFluor555 | ITS_rhCD45 | PR-0059
Target: CD45
Reactivity: Rhesus Macaque (Macaca mulatta)
Applications: in vivo in vivo; SIVS
Description:

The Anti-CD45 [ITS_rhCD45] antibody clone was developed for leukocyte trafficking studies using the Serial Intravascular Staining (SIVS) method (see references below). This mouse antibody clone specific to rhesus CD45 glycoprotein was produced from CHO cell lines, purified using protein A chromatographic separation, and conjugated to the Alexa Fluor dye (NHS chemistry).

 

SIVS Infusion protocols and research articles:

 

  1. Potter, E. and Roederer, M. (2022). αCD45 infusions. Bio-protocol. bio-protocol.org/prep1586
  2. Anderson, K., Mayer-Barber, K., Sung, H. et al. Intravascular staining for discrimination of vascular and tissue leukocytes. Nat Protoc 9, 209–222 (2014). https://doi-org.umassmed.idm.oclc.org/10.1038/nprot.2014.005
  3. Potter, E., Gideon, H., Tkachev, V., Fabozzi, G., Chassiakos, A., Petrovas, C., Darrah, P., Lin, P., Foulds, K., Kean, L., Flynn, J. and Roederer, M.(2021). Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining. Science Translational Medicine 13(576). DOI: 10.1126/scitranslmed.abb4582
Modifications: AF555
Restrictions: No
Sponsor: NIAID
How to cite (Methods): Anti-CD45 [ITS_rhCD45]-AF555 (NHPRR Cat#PR-0059, RRID:AB_2910538)
How to cite (Acknowledgments): The Anti-CD45 [ITS_rhCD45]-AF555 antibody used in this study was provided by the NIH Nonhuman Primate Reagent Resource (NIAID U24 AI126683).

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The Nonhuman Primate Reagent Resource (NHPRR) is a nonprofit laboratory established in 1999, and supported by the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), and Office of Research Infrastructure Programs (ORIP).

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